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A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms

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dc.affiliation.dptoUC3M. Departamento de Bioingenieríaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Biomedical Imaging and Instrumentation Groupes
dc.contributor.authorRipoll Lorenzo, Jorge
dc.contributor.authorRieckher, Matthias
dc.contributor.authorKyparissidis-Kokkinidis, Ilias
dc.contributor.authorZacharopoulos, Athanasios
dc.contributor.authorKourmoulakis, Georgios
dc.contributor.authorZacharakis, Giannis
dc.contributor.authorTavernarakis, Nektarios
dc.date.accessioned2015-05-26T07:33:17Z
dc.date.available2015-05-26T07:33:17Z
dc.date.issued2015-05-22
dc.description.abstractWe describe a customizable and cost-effective light sheet microscopy (LSM) platform for rapid three-dimensional imaging of protein dynamics in small model organisms. The system is designed for high acquisition speeds and enables extended time-lapse in vivo experiments when using fluorescently labeled specimens. We demonstrate the capability of the setup to monitor gene expression and protein localization during ageing and upon starvation stress in longitudinal studies in individual or small groups of adult Caenorhabditis elegans nematodes. The system is equipped to readily perform fluorescence recovery after photobleaching (FRAP), which allows monitoring protein recovery and distribution under low photobleaching conditions. Our imaging platform is designed to easily switch between light sheet microscopy and optical projection tomography (OPT) modalities. The setup permits monitoring of spatio-temporal expression and localization of ageing biomarkers of subcellular size and can be conveniently adapted to image a wide range of small model organisms and tissue samples.en
dc.description.sponsorshipMR, GZ and AZ acknowledge funding from the Projects “Skin-DOCTor” Grant No. 1778 and “Neureka!” Grant No. LSF7-341 implemented under the "ARISTEIA" and "Supporting Postdoctoral Researchers" Actions respectively, of the "OPERATIONAL PROGRAMME EDUCATION AND LIFELONG LEARNING", (http://www.espa.gr/en/pages/staticOPEducationandLifelongLearning.aspx), which is co-funded by the European Social Fund and National Resources and from the EU Marie Curie Initial Training Network “OILTEBIA”, Grant No. PITNGA-2012-317526 (http://ec.europa.eu/research/mariecurieactions/, http://gdo.uc3m.es/oiltebia/index. php?option = com_content&view = frontpage). JR acknowledges support from EC FP7 CIG grant HIGHTHROUGHPUT TOMO PICIG12-GA-2012-333632, (http://cordis.europa.eu/projects/333632), and Spanish MINECO grant MESO-IMAGING FIS2013-41802-R, (https://sede.micinn.gob.es/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
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dc.format.mimetypeapplication/pdf
dc.identifier.bibliographicCitationRieckher M, Kyparissidis-Kokkinidis I, Zacharopoulos A, Kourmoulakis G, Tavernarakis N, Ripoll J, et al. (2015) A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms. PLoS ONE 10(5): e0127869. doi:10.1371/journal.pone.0127869es
dc.identifier.doi10.1371/journal.pone.0127869
dc.identifier.issn1932-6203
dc.identifier.publicationfirstpage1
dc.identifier.publicationissue5
dc.identifier.publicationlastpage15
dc.identifier.publicationtitlePloS onees
dc.identifier.publicationvolume10
dc.identifier.urihttps://hdl.handle.net/10016/20814
dc.identifier.uxxiAR/0000016872
dc.language.isoeng
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/333632
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/317526
dc.relation.projectIDGobierno de España. FIS2013-41802-R
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0127869
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/
dc.subject.ecienciaMedicinaes
dc.titleA Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organismses
dc.typeresearch article*
dc.type.hasVersionVoR*
dspace.entity.typePublication
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